Job no: 494563
Position type: Staff - Full-Time
Location: Health Science Campus College
Division/Equivalent: College of Medicine
School/Unit: Med Microbio & Immunology - 107520
Categories: Post Doc
Title: Post Doctoral
Department Org: Med Microbio & Immunology - 107520
Employee Classification: P3 - Post Doctoral Assoc FT-HSC
Bargaining Unit: Bargaining Unit Exempt
Primary Location: Health Science Campus College
Posted Salary: Salary will commensurate with education and experience
On Call: False
The University of Toledo College of Medicine and Life Sciences is looking for two Postdoctoral fellows in the Department of Medical Microbiology and Immunology to work on National Institutes of Health (NIH) and EPA - funded projects study innate immune responses, particularly the interferon system, to protect against viral and non-viral diseases. The laboratory is interested in studying how the interferon system protects against virus infection. In this context, we are focusing on the key transcription factor Interferon Regulatory Factor 3 (IRF3), whose activities are required to inhibit viral replication in vitro and in vivo. We have shown that in addition to performing transcriptional functions, IRF3 also triggers direct apoptosis in virus-infected cells and the apoptotic pathway is critical for its antiviral activity (Glanz et al, Viruses 2020, Chattopadhyay et al Immunity 2016, J. Virol 2013, 2011, EMBO J 2010). We are interested to study, using knockout and knock-in mouse models, how this pathway regulates viral or non-viral diseases. IRF3, upon activation by virus infection, transcriptionally induces many antiviral genes, such as interferons and interferon stimulated genes (ISGs) (Chattopadhyay et al EMBO Rep 2015, mBio 2013). The protein products of these ISGs inhibit specific stages of viral life cycle, thereby inhibiting virus replication. Because all viruses cannot be inhibited by a single ISG, it is important to investigate virus-specific ISGs. Using high throughput screening approaches, we began to identify novel viral restriction mechanisms (Subramanian et al, JBC 2020, PLOS Pathogens, 2018). These mechanisms will be tested in vivo using appropriate viral pathogenesis models. Currently, we have several ongoing projects in our laboratory to study both pathways of IRF3 in viral and non-viral pathogenesis. Interested candidates should apply to this link below. Salaries and compensation will be based on first-authored publications and experience in the research area of our laboratory.
Ideal applicants are expected to be highly organized, detail-oriented, and able to perform multiple research activities (multi-task) on a daily basis. Occasional weekend and/or night laboratory work may be necessary.
Working experience with maintaining mouse colony, breeding transgenic mice, genotyping and functional characterization using such techniques as PCR, cloning, recombinant protein expression and purification, SDS-PAGE, western blotting, flow cytometry, fluorescence microscopy, and experience growing bacteria.
Ph.D. degree in biological/medical sciences (e.g., microbiology, molecular biology, biology, biochemistry, immunology) required.
Minimum five years laboratory research experience with either animal models or molecular biology techniques is required. Experience with both is strongly preferred.
Basic electronic word processing, database management, and data analysis skills are required.
Applicant must be able to clearly speak and write technical data in English and have the ability to work independently.
Principal Duties and Responsibilities
(List in order of importance) List of Job Duties (Start each duty with an action verb such as “operate,” “repair,” “perform,” “manage,” or “coordinate,” etc.) Percent of Time
1. Plan, perform and interpret microbiology, molecular biology, biochemistry, cell biology, and immunology laboratory experiments in a biosafety level (BSL)-2 and/or 3 environments, working with animal models (mouse or rat) of virus infection or other pathogens. Will independently and collaboratively perform assigned experiments. May be required to provide training to pre-doctoral students and post-doctoral fellows.
2. Genetic characterization of offspring and perform procedures on mice, including but not limited to injections, necropsies, and tissue collection. 20%
3. General laboratory maintenance and day-to-day operations, including media/solution preparation, autoclave sterilization, ordering and maintaining laboratory supplies, maintaining records (written and electronic), cleaning laboratory glassware/containers, and other research-associated duties as assigned. 5%
4. Organize, analyze and interpret research data. Compile results and report findings to PI for presentation at group meetings, progress reports to funding agency, and prepare manuscripts for publication. 25%
Conditions of Employment:
Equal Employment Opportunity Statement:
The University of Toledo is an equal opportunity, affirmative action employer. The University of Toledo does not discriminate in employment, educational programs, or activities on the basis of race, color, religion, sex, age, ancestry, national origin, sexual orientation, gender identity and expression, military or veteran status, disability, familial status, or political affiliation.
The University is dedicated to the goal of building a culturally diverse and pluralistic faculty and staff committed to teaching and working in a multicultural environment and strongly encourages applications from women, minorities, individuals with disabilities, dual-career professionals and covered veterans.
The University of Toledo provides reasonable accommodation to individuals with disabilities. If you require accommodation to complete this application, or for testing or interviewing, please contact the Human Resources Department at 419-530-4747 between the hours of 8:30AM and 5:00PM or apply online for an accommodation request.
Computer access is available at most public libraries and at the Office of Human Resources located in the Center for Administrative Support on the Main Campus of the University of Toledo.
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